Emmanuelle Rohrbach

Emmanuelle Rohrbach

Contact

Send mailTel. +41 (0) 58 765 6848

Centre Ecotox
GR B0 391
EPFL ENAC IIE-GE
Station 2
1015 Lausanne

Tasks at the Ecotox Centre

  • Responsible for the organisation and management of the laboratory (maintenance, stocks of consumables, orders)
  • Maintenance of the cultures of organisms for bioassays with soil and sediment
  • Participation and technical support for bioassays with soil and sediment samples (including the development of molecular biomarkers)
  • Participation and technical support in field work
  • Laboratory safety officer (toxic waste management)

Areas of Expertise

  • Bioassays
  • Molecular biology (DNA extraction, RNA extraction, PCR, Sanger sequencing, metatranscriptomics, 16S RNA Gene Amplicon libraries for the Illumina MiSeq system, cloning)
  • Microbiology
  • Laboratory safety officer (CoSECS)
  • Trainer of apprentices in biology

CV

  • since 2022

    Lab technician at the Ecotox Centre in Lausanne (60%)

  • since 2000

    Lab Technician at the EPFL- laboratoire de Biotechnologie environnementale – Professor C. Holliger (currently 40%)

  • 2013 - 2022

    Lab technician at the EPFL- Central environnemental molecular biology laboratory – Dr P. Rossi

  • 1998 - 1999

    Lab techncian - University of Geneva – Department of molecular biology - Dr E. Izaurralde

  • 1995 - 1998

    Lab technician at Glaxo-Wellcome GBRI – Genève plan-Les-Ouates (protein purification (biochemistry), research)

  • 1993 - 1995

    Lab technician at Ares SERONO in Aubonne, R&D and support of production

  • 1990 - 1993

    Apprenticeship with mention at Glaxo-Wellcome

Publications

Multistability and Reversibility of Aerobic Granular Sludge Microbial Communities Upon Changes from Simple to Complex Synthetic Wastewater ans Back. Aline Adler and Christof Holliger, Frontiers in Microbiology, 11, 574361 (2020), (acknowledgments Emmanuelle Rohrbach)

Temporal evolution of bacterial communities associated with the in-situ wetland-based remediation of a marine shore porphyry copper tailings deposit. N. Diaby, B. Dold, E. Rohrbach, C. Holliger and P. Rossi, in Science of the Total Environment, vol. 533, p. 110–121, 2015.

Use of silicate minerals for long-term pH control during reductive dechlorination of high tetrachloroethene concentrations in continuous flow-through columns. E. Lacroix, A. Brovelli, J. Maillard, E. Rohrbach-Brandt, D. A. Barry and C. Holliger, in Science of the Total Environment, vol. 482-483, p. 23-35, 2014.

Diversity of cobalamin riboswitches in the corrinoid-producing organohalide respirer Desulfitobacterium hafniense. P. K. Choudhary, A. Duret, E. Rohrbach-Brandt, C. Holliger, R. K. O. Sigel and J. Maillard, in Journal of bacteriology, vol. 195 (22), p. 5186-5195, 2013.

An unusual tandem-domain rhodanese harbouring two active sites identified in Desulfitobacterium hafniense. L. Prat, J. Maillard, E. Rohrbach-Brandt and C. Holliger, in Febs Journal, vol. 279, p. 2754-2767, 2012.

Reductive dechlorination of tetrachloroethene by a stepwise catalysis of different organohalide respiring bacteria and reductive dehalogenases. J. Maillard, M.-P. Charnay, C. Regeard, E. Rohrbach-Brandt, K. Rouzeau-Szynalski, P. Rossi and C. Holliger in Biodegradation, vol. 22, num. 5, p. 949-960, 2011.

Performance and microbial community composition dynamics of aerobic granular sludge from sequencing batch bubble column reactors operated at 20, 30, and 35°. S. Ebrahimi, S. Gabus, E. Rohrbach-Brandt, M. Hosseini, P. Rossi, J. Maillard and C. Holliger in Applied Microbiology and Biotechnology, vol. 87, p. 1555-1568, 2010.

Statistical assessment of the variability of the T-RFLP analysis applied to complex microbial communities. P: Rossi, F. Gillet, E. Rohrbach and C. Holliger in Applied and Environmental Microbiology, vol. 75, num. 22, p. 7268-7270, 2009.

TAP binds to the constitutive transport element (CTE) through a novel NA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus, I. C. Braun, E. Rohrbach, C. Schmitt, E. Izaurralde.

A bacterial signal peptide directs efficient secretion of Eucaryotic proteins in the Baculovirus, B. Allet, A. R. Bernard, A. Hochmann, E. Brandt- Rohrbach, p. Graber, E. Magnenat, G. Mazzei, L. Bernasconi, in Protein Expression and Purification 9, 61-68 (1997)

Expression and characterization of human D4 dopamine receptors in baculovirus-infected insect cells, A. Mills, B. Allet, A. Bernard, C. Chabert, E. Rohrbach-Brandt, C. Cavegn, A. Chollet, E. Kawashima: in Elsevier Science Publishers B.V., 130 (1993)