Test with Luminescent Bacteria
- Marine luminescent bacterium (Vibrio fischeri)
Detectable effects (impact)
- Inhibition of bioluminescence (unspecific toxicity)
- Based on the inhibition of the enzyme luciferase, which oxidises luciferin to generate light. Positive results indicate that a substance interferes with the energy metabolism of the cell.
- Effects of chemicals or environmental samples on the bioluminescence of the bacteria are measured after an exposure time of 30 min.
- approx. 0.5 days (exposure time: 30 min)
- Screening test for the toxicity of unknown samples
- Standard test organism for water quality evaluation (e.g. OECD, ISO, US EPA)
- Common in wastewater analyses and partly stipulated in ordinances (e.g. German Wastewater Ordinance)
Guidelines and literature
- International Organization for Standardization (2007). Water quality -- Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) -- Part 3: Method using freeze-dried bacteria. EN ISO 11348-3.
- Escher BI, Bramaz N, Mueller JF, Quayle P, Rutishauser S, Vermeirssen ELM (2008). Toxic equivalent concentrations (TEQs) for baseline toxicity and specific modes of action as a tool to improve interpretation of ecotoxicity testing of environmental samples. J. Environ. Monit. 10, 612-621.